泽泻汤提取物三萜类化学成分分析及其抗非酒精性脂肪肝作用研究

目的:对泽泻汤不同极性溶剂提取物三萜类化学成分进行定性分析并探究其对非酒精性脂肪肝的干预作用。方法:制备泽泻汤水及95%乙醇提取物,利用超高效液相色谱串联高分辨质谱技术对三萜类成分进行定性鉴别,并比较两种提取物中三萜类化合物数量及含量差异。采用油酸诱导的HepG2细胞脂肪累积模型评价泽泻汤不同极性提取物干预非酒精性脂肪肝的能力。结果:在泽泻汤中共鉴定20个三萜类色谱峰,水提取物中鉴定出14个三萜类成分,其中16-oxo-alisol A相对含量最高,95%乙醇提取物中鉴定出16个三萜类成分,alisol B 23-acetate相对含量最高。两提取物均能显著降低细胞中的脂肪累积量,且乙醇提取物表现出更强的抑制细胞内脂肪累积的作用。结论:泽泻汤水及95%乙醇提取物中三萜类化合物数量和含量存在差异,两种提取物均具有潜在抗非酒精性脂肪肝作用,且乙醇提取物活性更强,研究结果为泽泻汤进一步的药效物质基础及药理活性研究提供了基础。

Identification of the Triterpenoids in the Extracts of ZeXie Decoction and Investigation of Their Effects on Nonalcoholic Fatty Liver

ABSTRACT Objective: To identify the triterpenoids in the extracts of ZeXie Decoction (ZXD) prepared using different polar solvents and investigate their effects on the prevention and treatment of non-alcoholic fatty liver disease (NAFLD). Methods: Different ZXD extracts were prepared using water and 95% ethanol respectively, and an UPLC-MS/MS method was developed for the qualitative analysis of the triterpenoids in the two extracts. Then, oleic acid induced lipid accumulation model in HepG2 cells was established to evaluate the effects of the two extracts on NAFLD. Results: A total of 20 peaks were identified in ZXD, with 14 triterpenoids were detected in water extract and 16 in 95% ethanol extract. 16-Oxo-alisol A and alisol B 23-acetate were triterpenoids showed the highest relative content in the two extracts, respectively. Both tested ZXD extracts resulted in a dose-dependent reduction in intracellular lipid accumulation, with the ethanol extract being superior to the aqueous extract. Conclusion: The differences in the quantities and the relative contents of triterpenoids in the water and 95% ethanol extracts were observed. Both extracts had potential therapeutic effect on NAFLD, and the ethanol extract showed stronger activity. These results may be helpful for further research on the effective substance and pharmacological activity of ZXD.