MCM6、MCM7、KIAA1522蛋白联合检测对非小细胞肺癌诊断及预后预测的临床价值研究

摘要 目的：筛选肺癌蛋白分子标志物，寻找可诊断及预测肺癌预后的蛋白标志物。方法：选择2014年8月~2019年7月于西安市第四医院确诊并进行肺部切除手术的非小细胞肺癌(non-small-cell lung Cancer，NSCLC)患者80例，采用免疫组织化学(immunohistochemistry，IHC)检测NSCLC患者肺癌组织标本和癌旁MCM2(Minichromosome maintenance protein2, 微小染色体维持蛋白2)、MCM5(Minichromosome maintenance protein5，微小染色体维持蛋白5)、MCM6(Minichromosome maintenance protein6，微小染色体维持蛋白6)、MCM7(Minichromosome maintenance protein7，微小染色体维持蛋白7)、KIAA1522和KIAA0317蛋白表达阳性率，探讨多蛋白联合检测对NSCLC诊断及预后预测的临床应用价值。结果：肺癌组织中MCM2、MCM5、MCM6、MCM7、KIAA1522和KIAA0317的阳性表达率均显著高于癌旁正常肺组织(P＜0.05)，其中MCM6、MCM7和KIAA1522在50 %以上；以MCM6、MCM7、KIAA15223蛋白联合检测肺癌组织，不同性别、不同年龄、类型和分期的NSCLC患者的联合蛋白阳性率无统计学差异(P＞0.05)，且蛋白阳性率均大于80 %；MCM7高表达较之低表达或不表达的病例，显著增加患者的死亡风险(P=0.000)。男性(P=0.031)、III~IV期患者(P＜0.001)、以及低分化程度(P=0.012)也是患者的不良预后因素，多因素回归分析显示，MCM7是一个独立的预测指标(P=0.000)， 与患者生存具有显著相关性，对预后有一定的预测作用。结论：NSCLC患者肺癌组织中MCM6、MCM7和KIAA1522呈高表达，三者联合检测对NSCLC的检测具有较高的准确性、敏感性和特异性，高水平的MCM7表达提示肺癌患者的不良预后。

A Study on the Diagnostic and Prognostic Prediction Value of Combined Detection of MCM6, MCM7, and KIAA1522 for Non-Small Cell Lung Cancer

ABSTRACT Objective: Screening for molecular markers of lung cancer proteins to find protein markers that can diagnose and predict the prognosis of lung cancer. Methods: Eighty patients with NSCLC diagnosed in the Fourth Hospital of Xi''an from August 2014 to July 2019 and undergoing lung resection were selected, IHC was used to detect the positive rate of MCM2, MCM5, MCM6, MCM7, KIAA1522 and KIAA0317, to explore the clinical value of combined detection of multiple proteins in the diagnosis and prognosis prediction of NSCLC. Results: The positive rates of MCM2, MCM5, MCM6, MCM7, KIAA1522 and KIAA0317 in lung cancer tissues were significantly higher than normal tissues adjacent to cancer (P<0.05), of which MCM6, MCM7 and KIAA1522 were more than 50 %. In the joint detection of lung cancer tissues, there was no statistically significant difference in the combined protein positive rate of NSCLC patients of different genders, different ages, types and stages (P>0.05), and the protein positive rate was more than 80%; MCM7 high expression was lower than low expression or not The expressed cases significantly increased the patient''s risk of death (P=0.000). Males (P=0.031), patients with stage III to IV (P<0.001), and poor differentiation (P=0.012) are also the poor prognostic factors of patients. Multivariate regression analysis shows that MCM7 is an independent predictor (P=0.000), which has a significant correlation with the survival of patients and has a certain predictive effect on prognosis. Conclusion: MCL6, MCM7 and KIAA1522 are highly expressed in lung cancer tissues of NSCLC patients. The combined detection of the three has high accuracy, sensitivity and specificity for the detection of NSCLC. The high level of MCM7 expression indicates poor prognosis of lung cancer patients.