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Structure and Dynamics of the Second CARD of Human RIG-I Provide Mechanistic Insights into Regulation of RIG-I Activation
Authors:Fabien Ferrage  Kaushik Dutta  Estanislao Nistal-Villán  Jenish R Patel  María T Sánchez-Aparicio  Pablo De Ioannes  Angeliki Buku  Gloria González Aseguinolaza  Adolfo García-Sastre  Aneel K Aggarwal
Institution:1. Ecole Normale Supérieure, Département de Chimie, UMR 7203 CNRS-UPMC-ENS, 24, rue Lhomond, 75005 Paris, France;2. Department of Structural and Chemical Biology, Mount Sinai School of Medicine, Box 1677, 1425 Madison Avenue, New York, NY 10029, USA;3. New York Structural Biology Center, 89 Convent Avenue, New York, NY 10027, USA;4. Department of Microbiology, Mount Sinai School of Medicine, 1468 Madison Avenue, New York, NY 10029, USA;5. Division of Infectious Diseases, Department of Medicine, Mount Sinai School of Medicine, 1468 Madison Avenue, New York, NY 10029, USA;6. Global Health and Emerging Pathogens Institute, Mount Sinai School of Medicine, 1468 Madison Avenue, New York, NY 10029, USA;7. Department of Hepatology and Gene Therapy, Centro de Investigación Medica Aplicada (CIMA), University of Navarra, Pamplona 31080, Spain
Abstract:
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  • Highlights? The structure of a CARD of human RIG-I ? The mechanism and structural role of phosphorylation are revealed ? In trans interaction with the Helicase and regulatory domains is shown ? Lys172 lies in proximity to the CARD2:helicase-CTD interface
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