两种赋形剂载体中姜黄素干预慢性应激大鼠抑郁样行为和肌肉炎性反应的作用研究

目的:从抑郁样行为和肌肉炎性反应角度,研究姜黄素在由可可脂和花生油两种赋形剂载体时,对慢性应激大鼠的干预作用。方法:分别制备以可可脂和花生油为赋形剂的姜黄素制剂,使用SD雄性大鼠50只随机分为空白对照组、模型对照组、姜黄素可可脂组、姜黄素花生油组、阳性对照组,除空白对照组外,各组均采用束缚、噪音声刺激、冰水浴刺激、夹尾疼痛刺激等方法持续6周建立慢性应激大鼠模型,在维持模型刺激条件的基础上分别给予各种设计药物,连续用药7 d后在考察各组模型动物悬尾挣扎时间、机械痛阈、糖水偏好的行为学变化,然后使用Elisa试剂盒法检测各组大鼠脑中5五羟色胺(5-HT)和多巴胺(DA)含量,制作大鼠咬肌组织的HE染色切片观察肌肉病理变化,检测咬肌组织中高迁移率族蛋白B1(HMGB-1)、白介素1β(IL-1β)、白介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平。结果:与模型组相比,两种姜黄素均能显著降低应激大鼠抑郁样行为,改善脑内5-HT和DA含量的作用较阳性对照组弱,咬肌病理切片中两种姜黄素组可明显减少应激大鼠咬肌组织血管周围的炎性反应。两种姜黄素能显著降低咬肌组织中的IL-1β、IL-6、TNF-α水平(P<0.05),其中姜黄素可可脂组作用最明显。结论:两种姜黄素制剂对抗慢性应激大鼠的抑郁样症状均有治疗作用,均可降低咬肌中炎性因子HMGB-1、IL-1β、IL-6、TNF-α水平,其中可可脂作为赋形剂时作用较显著,在姜黄素的抗抑郁制剂研究中具有较好的开发潜力。

Effect of Curcumin in Two Carriers on Depression Behavior and Muscle Inflammatory Response in Chronic Stress Rats

Objective: From the perspective of depression-like behavior and muscle inflammatory response, the curative effect of curcumin in two excipient carriers of cocoa butter and peanut oil on chronic stress rats was studied. Methods: Curcumin preparations were prepared with cocoa butter and peanut oil as excipients respectively, and 50 male SD rats were randomly divided into blank control group, model control group, curcumin cocoa butter group, curcumin peanut oil group, and positive control group. Except for the blank control group, each group used the methods of restraint, noise and sound stimulation, ice water bath stimulation, tail pain stimulation, etc.for 6 weeks to establish a chronic stress rat model. Each was given various design drugs based on maintaining the model stimulation conditions. After 7 days of continuous medication, the behavioral changes of the tail suspension struggling time, mechanical pain threshold,and sugar water preference of model animals in each group were investigated, and then use the Elisa kit method to detect the content of5-HT and DA in the brain of each group of rats. Make HE-stained rat masseter tissue sections, observe the pathological changes of muscles, and detecte the levels of HMGB-1, IL-1β, IL-6, and TNF-α in masseter muscle. Results: Compared with the model group, both curcumin preparations can significantly reduce the depression-like behavior of stress rats and improve the content of 5-HT and DA in the brain. Compared with the positive control group, the two curcumin in the masseter muscle pathology section group can significantly reduce the inflammatory response around the masseter muscle vessels in stress rats. The two curcumin can significantly reduce the levels of IL-1β, IL-6 and TNF-α in masseter muscle tissue(P<0.05), and the curcumin cocoa butter group had the most obvious effect. Conclusions: Both curcumin preparations have a therapeutic effect against depression-like symptoms in chronic stress rats, and can reduce the levels of inflammatory factors HMGB-1, IL-1β, IL-6, and TNF-α in masseter muscle. Cocoa butter has a significant effect when used as an excipient, and it has good development potential in the research of curcumin’s antidepressant preparations.