Comparative proteomic analysis of seedling leaves of different salt tolerant soybean genotypes |
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Authors: | Ma Hongyu Song Liru Shu Yingjie Wang Shuang Niu Juan Wang Zhankui Yu Tian Gu Weihong Ma Hao |
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Affiliation: | a State Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR Chinab Animal and Plant Introduction and Research Center, Shanghai Agricultural Academy, Shanghai 201106, PR China |
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Abstract: | Salinity is one of the major environmental constraints limiting yield of crop plants in many semi-arid and arid regions around the world. To understand responses in soybean seedling to salt stress at proteomic level, the extracted proteins from seedling leaves of salt-sensitive genotype Jackson and salt-tolerant genotype Lee 68 under 150 mM NaCl stress for 1, 12, 72 and 144 h, respectively, were analyzed by 2-DE. Approximately 800 protein spots were detected on 2-DE gels. Among them, 91 were found to be differently expressed, with 78 being successfully identified by MALDI-TOF-TOF. The identified proteins were involved in 14 metabolic pathways and cellular processes. Based on most of the 78 salt-responsive proteins, a salt stress-responsive protein network was proposed. This network consisted of several functional components, including balancing between ROS production and scavenging, accelerated proteolysis and reduced biosynthesis of proteins, impaired photosynthesis, abundant energy supply and enhanced biosynthesis of ethylene. Salt-tolerant genotype Lee 68 possessed the ability of higher ROS scavenging, more abundant energy supply and ethylene production, and stronger photosynthesis than salt-sensitive genotype Jackson under salt stress, which may be the major reasons why it is more salt-tolerant than Jackson. |
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Keywords: | AAS, aspartate aminotransferase ACO, 1-aminocyclopropane-1-carboxylate oxidase ACC, 1-aminocyclopropane-1-carboxylate ACS, 1-aminocyclopropane-1-carboxylate synthetase Ald, aldolase APX, ascorbate peroxidase ASD, aspartate-semialdehyde dehydrogenase ATP, adenosine-triphosphate BIP, 2,3-biphosphoglycerate-independent phosphoglycerate mutase, putative CAT, catalase CMD, cytosolic malate dehydrogenase DFR, dihydroflavonol reductase EF2, elongation factor 2 EF-G, translation elongation factor EF-G G3PD, glyceraldehyde 3-phosphate dehydrogenase Glyox, putative glyoxalase GRP, glycine-rich RNA-binding protein HPD, homologous to plastidic aldolase KEGG, kyoto encyclopedia of genes and genomes NCBI, national center for biotechnology information OEEP2, oxygen-evolving enhancer protein 2, chloroplastic OX, oxidation PB, protein biosynthesis PDH, pyruvate dehydrogenase PDI, protein disulfide isomerase-like Perox, peroxiredoxin POD, peroxidase PP, proteolytic proteins PS I, photosystem I PS II, photosystem II RBPs, RNA-binding proteins RED, reduction RLS, ribulose 1,5-bisphosphate carboxylase large subunit RV, relative volume SAMS, S-adenosylmethionine synthetase SOD, superoxide dismutase TatBP, rice homologue of Tat binding protein TCA cycle, tricarboxylic acid cycle TCTP, translationally-controlled tumor protein Trans, transketolase. |
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