Attenuated metabolism is a hallmark of obesity as revealed by comparative proteomic analysis of human omental adipose tissue |
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Authors: | Pérez-Pérez Rafael García-Santos Eva Ortega-Delgado Francisco J López Juan A Camafeita Emilio Ricart Wifredo Fernández-Real José-Manuel Peral Belén |
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Institution: | a Instituto de Investigaciones Biomédicas, Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC) & Universidad Autónoma de Madrid, E-28029 Madrid, Spainb CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN) ISCIII, Spainc Department of Diabetes, Endocrinology and Nutrition, Hospital Dr. Josep Trueta, E-17007 Girona, Spaind Unidad de Proteomica, Centro Nacional de Investigaciones Cardiovasculares (CNIC), E-28029 Madrid, Spain |
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Abstract: | Obesity is recognized as an epidemic health problem worldwide. In humans, the accumulation of omental rather than subcutaneous fat appears to be tightly linked to insulin resistance, type 2 diabetes and cardiovascular disease. Differences in gene expression profiles in the adipose tissue comparing non-obese and obese subjects have been well documented. However, to date, no comparative proteomic studies based on omental fat have investigated the influence of obesity in protein expression. In this work, we searched for proteins differentially expressed in the omental fat of non-obese and obese subjects using 2D-DIGE and MS. Forty-four proteins, several of which were further studied by immunoblotting and immunostaining analyses, showed significant differences in the expression levels in the two groups of subjects. Our findings reveal a clearly distinctive proteomic profile between obese and non-obese subjects which emphasizes: i) reduced metabolic activity in the obese fat, since most down-regulated proteins were engaged in metabolic pathways; and ii) morphological and structural cell changes in the obese fat, as revealed by the functions exerted by most up-regulated proteins. Interestingly, transketolase and aminoacylase-1 represent newly described molecules involved in the pathophysiology of obesity, thus opening up new possibilities in the study of obesity. |
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Keywords: | 2D-DIGE MALDI-MS Obesity Human adipose tissue TKT ACY-1 |
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