Isolation of human nasoseptal chondrogenic cells: a promise for cartilage engineering |
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Authors: | do Amaral Ronaldo J F C Pedrosa Carolina da S G Kochem Michele C L Silva Karina R da Aniceto Marcelo Claudio-da-Silva Cesar Borojevic Radovan Baptista Leandra S |
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Institution: | a Programa de Pós-graduação em Ciências Morfológicas, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazilb Programa de Bioengenharia, Diretoria de Programas, Instituto Nacional de Metrologia Normalização e Qualidade Industrial (Inmetro), Duque de Caxias (RJ), Brazilc Programa de Pós-graduação em Clínica Médica, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazild Serviço de Cirurgia Plástica, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (RJ), Brazile Universidade Federal do Rio de Janeiro (UFRJ), Polo Xerém, Duque de Caxias (RJ), Brazil |
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Abstract: | In cartilaginous tissues, perichondrium cambium layer may be the source of new cartilage. Human nasal septal perichondrium is considered to be a homogeneous structure in which some authors do not recognize the perichondrium internal zone or the cambium layer as a layer distinct from adjacent cartilage surface. In the present study, we isolated a chondrogenic cell population from human nasal septal cartilage surface zone. Nasoseptal chondrogenic cells were positive for surface markers described for mesenchymal stem cells, with exception of CD146, a perivascular cell marker, which is consistent with their avascular niche in cartilage. Although only Sox-9 was constitutively expressed, they also revealed osteogenic and chondrogenic, but not adipogenic, potentials in vitro, suggesting a more restricted lineage potential compared to mesenchymal stem cells. Interestingly, even in absence of chondrogenic growth factors in the pellet culture system, nasoseptal chondrogenic cells had a capacity to synthesize sulfated glycosaminoglycans, large amounts of collagen type II and to a lesser extent collagen type I. The spontaneous chondrogenic potential of this population of cells indicates that they may be a possible source for cartilage tissue engineering. Besides, the pellet culture system using nasoseptal chondrogenic cells may also be a model for studies of chondrogenesis. |
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