ADP-ribosylation of isolated rat islets of Langerhans |
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Authors: | C Bernofsky D G Amamoo |
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Institution: | Laboratory of Molecular Biology Mayo Foundation and Mayo Graduate School of Medicine Rochester, Minnesota 55901 USA |
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Abstract: | A rapid and reproducible radioimmunoassay method was developed for rat atrial natriuretic factor (ANF)-IV. The method is also applicable to human atrial peptide. ANF was detected in rat hypothalamus (5.03 pmoles/g tissue), right (86.8 pmoles/mg tissue) and left atria (52.5 pmoles/mg tissue), and plasma (156 fmoles/ml). After high salt intake immunoreactive ANF in atria and plasma increased significantly, while a significant decrease was observed in hypothalamus. Gel chromatography revealed high and low molecular weight ANF in atria and hypothalamus while only a low molecular weight form was found in plasma. |
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Keywords: | To whom requests for reprints should be directed Present address: Department of Biochemistry Tulane University School of Medicine New Orleans Louisiana 70112 |
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