Expression and purification of CB2 for NMR studies in micellar solution |
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| Authors: | Krepkiy Dmitriy Gawrisch Klaus Yeliseev Alexei |
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| Affiliation: | National Institute on Alcoholism and Alcohol Abuse, National Institutes of Health, Bethesda, MD 20892-9410, USA. |
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| Abstract: | We demonstrate feasibility of biophysical characterization of the peripheral cannabinoid receptor CB2 produced by heterologous expression in E. coli membranes. Recombinant receptor was purified by affinity chromatography, and NMR diffusion experiments performed on CB2 solubilized in dodecylphosphocholine (DPC) micelles. Circular dichroism spectroscopy indicated high alpha-helical content (49 %) of CB2. |
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