In vitro selection and identification of sweetpotato (<Emphasis Type="Italic">Ipomoea batatas</Emphasis> (L.) Lam.) plants tolerant to NaCl |
| |
Authors: | Shaozhen He Yuanfeng Han Yuping Wang Hong Zhai Qingchang Liu |
| |
Institution: | (1) Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, China Agricultural University, Beijing, 100193, China;(2) Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China;(3) Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing, 100193, China |
| |
Abstract: | In vitro selection of sweetpotato (Ipomoea batatas (L.) Lam.) plants tolerant to NaCl was achieved using embryogenic suspension cultures of sweetpotato cv. Lizixiang and gamma-ray
induced mutation. Cell aggregates from embryogenic suspension cultures of Lizixiang were irradiated with 80 Gy gamma-ray,
and 1 week after irradiation they were cultured in a selective medium containing 342 mM NaCl for in vitro selection. A total
of 276 plants were regenerated from the irradiated 2,783 cell aggregates by a two-step in vitro selection procedure. After
the regenerated plants were propagated into plant lines on the basal medium, they were cultured on the medium supplemented
with 86, 171, 257 and 342 mM NaCl, respectively, in order to evaluate their in vitro salt tolerance. Of them 18 plant lines
showed significantly higher in vitro salt tolerance than control plants. Proline and superoxide dismutase (SOD) were more
accumulated in these 18 plant lines than in control plants when both were exposed to NaCl. Salt tolerance of the 18 plant
lines was further evaluated with Hoalgland solution containing different concentrations of NaCl in a greenhouse. The results
indicated that 3 of them had significantly better growth and rooting ability than the remaining 15 plant lines and control
plants at 171 mM NaCl. |
| |
Keywords: | Embryogenic suspension cultures Gamma-ray induced mutation In vitro selection NaCl tolerance Sweetpotato |
本文献已被 SpringerLink 等数据库收录! |
|