2-O-methyl-d-xylose containing sheath in the cyanobacterium Gloeothece sp. PCC 6501 |
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Authors: | J. Weckesser C. Broll S. P. Adhikary U. J. Jürgens |
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Affiliation: | (1) Institut für Biologie II, Mikrobiologie, der Albert-Ludwigs-Universität, Schänzlestr. 1, D-7800 Freiburg, Federal Republic of Germany;(2) Present address: Dharanidhar College, 758001 Keonjhar, Orissa, India |
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Abstract: | The sheath of the unicellular cyanobacterium Gloeothece sp. PCC 6501 was isolated from cell homogenates by differential and sucrose gradient centrifugation, followed by lysozyme treatment and hot sodium dodecyl sulfate extraction. The sheath contains a major fraction of carbohydrate consisting of galactose, glucose, mannose, rhamnose, 2-O-methyl-d-xylose, xylose, glucuronic and galacturonic acids, but only traces of fatty acids and phosphate. A protein content of about 2% (of fraction dry weight) could not be removed by the detergent treatment.Abbreviation SDS sodium dodecyl sulfate |
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Keywords: | Cyanobacteria Gloeothece 2-O-Methyl- font-variant:small-caps" >d-xylose Polysaccharide Sheath |
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