|
|||||
|
|
Filamentous vibriophage fs2 encoding the rstC gene integrates into the same chromosomal region as cholera toxin X |
|
| Authors: | Dong Tu Nguyen Binh Minh Nguyen Huy Hoang Tran Tuan Cuong Ngo Thanh Huong Le Hoai Thu Nguyen M John Albert Mamoru Iwami & Masahiko Ehara |
| Institution: | Department of Bacteriology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam;;Department of Microbiology, Faculty of Medicine, Kuwait University, Safat, Kuwait;;and Department of Bacteriology, Institute of Tropical Medicine, Nagasaki University, Japan |
| Abstract: | The genome of the filamentous phage of Vibrio cholerae fs2 was found to contain rst C and rst B1 (truncated) genes downstream of ORF500. att -fs2-dir and att- fs2-rev sequences homologous to that of att -CTXφ were found between orf 500 and rst C of the fs2 genome. This prompted us to search for the integration site of fs2 in the genomes of V. cholerae O1 and O139. The genome of fs2 was found to integrate downstream of att RS of the CTXφ phage, which integrated into chromosome I of V. cholerae O1 and O139. When infected with fs2, a fimbriate strain of V. cholerae O1 appeared to reduce fimbrial production in an adult rabbit ileal loop assay. |
| Keywords: | filamentous phage fs2 Vibrio cholerae O1 and O139 rstC site-specific integration |