Partial recovery of cell membrane-bounded human interleukin-2 fusion protein from insect cell debris by using various detergent extractions |
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| Authors: | Hye Sook Cho Hwa Sung Shin William E. Bentley Hyung Joon Cha |
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| Affiliation: | (1) Department of Chemical Engineering & Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, 790-784, Korea;(2) Department of Chemical Engineering & Center for Agricultural Biotechnology, University of Maryland, College Park, Maryland 20742, USA |
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| Abstract: | We previously found that the human interleukin-2 (hIL-2) fused with green fluorescent protein (GFP) mainly remained in the insect cell debris after disruption due to the highly hydrophobic property of hIL-2 itself. Even though the significant GFPuv/hIL-2 fusion proteins were associated with cell membrane fractions, these were still functionally active. Therefore, to increase the total product yield, we performed partial recovery of the cell membrane-bounded hIL-2 fusion protein from the insoluble cell debris using several non-ionic, zwitterionic, and anionic detergents. |
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| Keywords: | detergent green fluorescent protein human interleukin-2 insect Tricoplusia ni larvae membrane protein |
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