Changes in Peroxidases and Catalase Activity During in vitro Rooting |
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Authors: | AN Molassiotis K Dimassi G Diamantidis I Therios |
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Institution: | (1) Laboratory of Pomology, School of Agriculture, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece;(2) Laboratory of Agricultural Chemistry, School of Agriculture, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece |
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Abstract: | Enzyme changes in non-rooted (treated with Fe-EDTA) and rooted (treated with Fe-EDDHA) stems of rootstock GF-677 (Prunus amygdalus×P. persica) during adventitious root formation in vitro have been recorded. The first roots appeared approximately after 12 d on the rooting medium. By contrast to non-rooted stems, rooted stems showed a maximum of soluble peroxidase activity on the 9th day, of ionically bound peroxidase to cell wall on the 6th and 12th day and of catalase on the 6th and the 15th day. A time course study of changes of soluble peroxidases isoenzymes showed that there was a band visible only in the rooted stems and also a new band appeared three days before the emergence of roots. |
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Keywords: | GF-677 (Prunus amygdalus× P persica) iron |
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