Double-strand DNA hydrolysis by dilanthanide complexes |
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Authors: | Mark E. Branum L. Que Jr. |
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Affiliation: | (1) Department of Chemistry and Center for Metals in Biocatalysis University of Minnesota, 207 Pleasant Street S.E., Minneapolis MN 55455, USA e-mail: que@chem.umn.edu Tel: +1-612-6250389, Fax: +1-612-6247029, US |
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Abstract: | Although there has been progress in developing artificial hydrolytic DNA cleaving agents, none of these has been shown to carry out the double-strand hydrolysis of DNA. We demonstrate that La(III) or Ce(IV) combined with the ligand 1,3-diamino-2-hydroxypropane-N,N,N′,N′-tetraacetate (HPTA) in a 2 : 1 ratio can efficiently cleave supercoiled plasmid DNA at 55 °C within a 3-h period. Analysis of end-labeled restriction fragments cleaved by these complexes reveals 3′- and 5′-ends consistent with a hydrolytic mechanism. Unlike for other polydentate carboxylate complexes, plasmid DNA cleavage by La2(HPTA) or Ce2(HPTA) affords a significant amount of linear DNA with a considerable fraction of the supercoiled form still remaining. This result implies that La2(HPTA) and Ce2(HPTA) can carry out double-strand cleavage of plasmid DNA. La2(HPTA) and Ce2(HPTA) represent the first metal complexes demonstrated to be capable of double-strand hydrolytic cleavage of plasmid DNA. Received: 29 March 1999 / Accepted: 9 July 1999 |
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Keywords: | DNA hydrolysis Double-strand DNA Cleavage Lanthanide complexes Restriction endonucleases |
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