Effect of experimental diabetes on glycolytic intermediates and regulation of phosphofructokinase in rat lens |
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Authors: | A M Gonzalez M Sochor P McLean |
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Affiliation: | Courtauld Institute of Biochemistry, The Middlesex Hospital Medical School, London, W1P 7PN, Great Britain. |
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Abstract: | The pattern of glycolytic intermediates in the lens of alloxan-diabetic rats was indicative of regulation at phosphofructokinase. The changes in metabolites influencing phosphofructokinase activity in the diabetic, relative to the normal, rat lens were: glucose 6-phosphate, 182%; fructose 6-phosphate, 107%; fructose diphosphate, 57%. There was also a marked decrease in phosphoenolpyruvate, pyruvate, lactate and ATP but no significant change in other triose phosphates or cyclic AMP. The resuts are considered in relation to the early changes in [Ca2+] known to occur in lens in diabetes and to the coordinating effect of fructose diphosphate on flux through the glycolytic route. |
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Keywords: | PFK phosphofructokinase ATP D-fructose 6-phosphate 1-phosphotransferase, EC 2.7.1.11) cyclic AMP adenosine 3′5′-monophosphate |
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