Osmotic control of luminescence and growth in Photobacterium leiognathi from ponyfish light organs |
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Authors: | Paul V Dunlap |
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Institution: | (1) Department of Biology, University of California at Los Angeles, 90024 Los Angeles, CA, USA;(2) Present address: Department of Microbiology, College of Agriculture and Life Sciences, Cornell University, 14853 Ithaca, NY, USA |
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Abstract: | Osmolarity was found to control the luminescence and growth of Photobacterium leiognathi strain LN-1a isolated from the light organ of the ponyfish Leiognathus nuchalis (family Leiognathidae). Low osmolarity (ca. 400 mOsm) stimulated luminescence per cell 80 to 100-fold to a level (ca. 2.0×104 quanta·s-1·cell-1) equal to that of bacteria taken directly from the light organ and increased the level of luciferase per cell 8 to 10-fold compared to high osmolarity (ca. 800 mOsm). Conversely, high osmolarity stimulated oxygen uptake and growth rate 2 to 4-fold compared to low osmolarity. Of 21 additional tested strains of P. leiognathi from light organs of 9 other ponyfish species, all responded similarly. Low osmolarity may be a host control factor that functions to stimulate the luminescence and restrict the growth of ponyfish light organ bacteria in situ. |
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Keywords: | Luminescence Luciferase Osmolarity Growth Ponyfish Light organ symbiosis Photobacterium leiognathi Salt |
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