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Multiplex RT-PCR with broad-range primers and an exogenous internal amplification control for the detection of honeybee viruses in bumblebees |
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| Authors: | Ivan Meeus Guy Smagghe Kris Jans |
| Institution: | a Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium b Laboratory of Zoophysiology, Department of Physiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium c Bieneninstitut Kirchhain, Landesbetrieb Landwirtschaft Hessen, Erlenstr. 9, D-35274 Kirchhain, Germany d Biobest NV, Ilse Velden 18, B-2260 Westerlo, Belgium |
| Abstract: | Bumblebees are commercially reared and transported worldwide mainly for pollination of greenhouse tomatoes. Three honeybee viruses have been reported in bumblebees: Acute bee paralysis virus, Kashmir bee virus and Deformed wing virus. We developed a multiplex RT-PCR with primers designed on highly conserved regions of the RNA-dependent RNA polymerase in order to detect a maximum range of viral variants. Rearing facilities and governmental organizations can now thoroughly screen bumblebee colonies with a cost-effective technique with an integrated internal amplification control (IAC) implementable in laboratories that strive for International Organization for Standardization (ISO) certification. |
| Keywords: | Acute bee paralysis virus Kashmir bee virus Deformed wing virus Multiplex RT-PCR RNA-dependent RNA polymerase Bumblebees |
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