Differentiation and inter-genomic relationships among C, E and D genomes in the Oryzaofficinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization |
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Authors: | C.-B. Li D.-M. Zhang S. Ge B.-R. Lu D.-Y. Hong |
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Affiliation: | (1) Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China e-mail: zhangdm@ns.ibcas.ac.cn, CN;(2) Genetic Resources Center, International Rice Research Institute (IRRI), MCPO Box 3127, 1271 Makati City, The Philippines, Current address: Institute of Biodiversity Science, Fudan University, Shanghai 200433, China, CN |
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Abstract: | The multicolor genomic in situ hybridization (McGISH) method was used to study differentiation and relationships among the C, D and E genomes in the officinalis complex of the genus Oryza. The chromosomes of Oryza alta (CCDD genomes) were hybridized with labelled probes of the C genome (from diploid Oryza eichingeri and Oryza officinalis) and the E genome (from Oryza australiensis) simultaneously. By adjusting the post-hybri- dization washing stringency in a gradual series, differentiation between the genomes was detected according to the homology between the target genomes and the probes. The McGISH results indicate that the C, D and E genomes share a substantial amount of similar sequences, and differentiation between the D and C genomes of O. alta is less than that between the E genome and each of the C and D genomes. The differentiation within the C genomes of the diploid species (O. officinalis and O. eichingeri) and the C genome of O. alta was clearly discerned by McGISH, suggesting strongly that neither O. officinalis nor O. eichingeri was the direct C-genome donor of O. alta. The evidence of the GISH results also indicates that the E genome was considerably differentiated from the C and D genomes. Therefore, the E genome should not be the direct donor of O. alta; on the contrary, the E genome is closer to the C than to the D genome. McGISH is an efficient method in revealing the relationships among the genomes in question, particularly under the gradual stringent-washing condition. Received: 14 February 2000 / Accepted: 14 November 2000 |
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Keywords: | Genomic in situhybridization (GISH) Oryza officinalis complex C genome E genome CD genome |
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