Quantitative reappraisal of general expressions for multivalent protein binding in subunit-exchange chromatography |
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| Authors: | P W Chun D E Milov W S Jou |
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| Institution: | Department of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville 32610. |
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| Abstract: | Quantitative expressions have been derived for bivalent equilibria with immobilized ligand systems and for the equilibria for an immobilized protein whose self-association is modified by binding with a soluble ligand, as analyzed by affinity chromatography. These general expressions have been applied in a reexamination of multivalency in the affinity chromatography of antibodies, as reported by Eilat and Chaiken (Biochemistry 18 (1979) 790) and also to studies of neurophysin-peptide hormone interactions using glass matrices reported by Swaisgood and Chaiken (Biochemistry 25 (1986) 4148). |
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