Restriction mapping of deletions in the nif region of the Klebsiella pneumoniae chromosome |
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Authors: | Edward R Appelbaum and Richard A Kramer |
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Institution: | (1) Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, 20205 Bethesda, Maryland, USA |
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Abstract: | Summary Chromosomal DNA restriction fragments carrying the nitrogen fixation (nif) and his genes of Klebsiella pneumoniae were identified in hybridization experiments using a plasmid derived from pRD1 as a radioactive probe. Restriction mapping of 26 genetically characterized chromosomal nif deletions provided a map showing the physical location of nif genes along the chromosome. |
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