Investigations on the biosynthesis of steroids and terpenoids: XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae |
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Authors: | Trevor R. Jarman A.A.Leslie Gunatilaka David A. Widdowson |
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Affiliation: | Chemistry Department, Imperial College, London SW7 2AY, England |
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Abstract: | The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis. |
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