Lectins of the <Emphasis Type="Italic">Nicotiana tabacum</Emphasis> pollen grain walls stimulating in vitro pollen germination |
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Authors: | N P Matveeva E A Lazareva T P Klyushnik S A Zozulya I P Ermakov |
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Institution: | (1) Department of Plant Physiology, Faculty of Biology, Moscow State University, Vorob’evy gory, Moscow, 119899, Russia |
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Abstract: | Proteins diffusing from tobacco pollen grains into external medium, being inactivated by low temperature (0°C), were shown to stimulate pollen germination in vitro. Fractionation of these proteins by affinity chromatography using α-D-methylmannopyranoside (MMP) immobilized on agarose resulted in the isolation of lectins stimulating germination. The mol wts of these lectins were estimated by SDS-PAGE as 58, 69, and 74 kD. A stimulatory effect of these lectins was determined by their specific interaction with carbohydrate determinants because a competitive sugar (0.3 M MMP) suppressed completely lectin effect on germination. Polyvalent lectins capable of erythrocyte agglutination were also found among diffused proteins. These lectins are glycoproteins with Glu/Man carbohydrate determinants. MMP did not affect their capability of agglutination. This finding permits a conclusion that pollen grain wall contains lectins differing in their carbohydrate specificity. |
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Keywords: | Nicotiana tabacum pollen grain pollen germination pollen wall lectins |
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