Aldosterone production after short-term culture of rat adrenal capsule: responsiveness to angiotensin II, potassium and ACTH |
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| Authors: | J H Pratt D A Turner J A McAteer |
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| Institution: | 1. Grupo Fisiopatología Endocrina, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS).;2. CIBER Fisiopatología Obesidad y Nutrición, Instituto de Salud Carlos III, Spain;3. Department of Physiology, Research Centre of Molecular Medicine and Chronic Diseases (CIMUS), Santiago de Compostela, Spain;4. Pediatric Department, Universidad de Santiago de Compostela (USC), Santiago de Compostela, Spain;5. Cellular and Molecular Cardiology Research Unit and Department of Cardiology, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain;6. Servicio de Cirugía General, Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain;7. Laboratorio de Endocrinología Molecular y Celular, Universidad de Santiago de Compostela (USC), Santiago de Compostela, Spain;8. Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain |
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| Abstract: | In vitro studies of aldosterone production have traditionally used freshly isolated adrenal glomerulosa tissue. In the present study we examined the effects of short-term culture of rat adrenal capsule on its secretory capacity by measuring both basal and stimulated aldosterone production. Capsules were maintained in culture for 24 h, and then responses to administered angiotensin II (1 X 10(-7) M), potassium (an increase of 2mM) and ACTH (1 X 10(-8) M) were determined during perifusion. Results were compared with responses by freshly isolated adrenal capsule. Although short-term culture reduced basal aldosterone production, responsiveness to administered stimuli was intact and often was greater than that observed with fresh capsular tissue. The results indicate that short-term culture of zona glomerulosa provides a suitable in vitro preparation for examining aldosterone secretory responsiveness to stimuli. |
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