siRNA沉默整合素beta1 基因对子宫内膜癌细胞侵袭、转移的影响

目的：探讨siRNA 沉默整合素茁1 基因对子宫内膜癌细胞侵袭、转移的影响。方法：选取子宫内膜癌ECC-1细胞系(ER阳性)和KLE细胞系(ER阴性),分别转染Integrin beta1 siRNA 质粒(Integrin beta1 siRNA 组)、无义序列siRNA质粒(无义序列对照组)和空载质粒(空载对照组),利用实时荧光定量PCR 检测各组细胞中Integrin 茁1 mRNA的表达,Western blot 检测各组细胞中Integrin beta1、beta-catenin 和C-Myc 蛋白的表达,Transwell 小室检测各组细胞迁移和侵袭能力,MTT 法检测各组细胞的增殖情况。结果：Integrinbeta1 siRNA 组ECC-1 细胞和KLE 细胞中Integrin beta1 mRNA 和蛋白相对表达量均低于无义序列对照组和空载对照组(P<0.05);Integrin beta 1 siRNA组ECC-1 细胞和KLE细胞中beta-catenin 蛋白和C-Myc 蛋白相对表达量均低于无义序列对照组和空载对照组,差异均有统计学意义(P<0.05);Integrin beta1 siRNA组ECC-1 细胞和KLE 细胞中迁移细胞数和侵袭细胞数均低于无义序列对照组和空载对照组(P<0.05);Integrin beta1 siRNA 组ECC-1细胞和KLE 细胞的A 值均低于无义序列对照组和空载对照组(P<0.05)。结论：特异性抑制Integrin beta1 基因可抑制子宫内膜癌细胞迁移、侵袭和增殖,可能与抑制Wnt信号传导有关。

Effects of siRNA Silencing Integrin beta1 Gene on the Invasion and Metastasisof Endometrial Carcinoma Cells

Objective:To investigate the effects of siRNA silencing integrin beta1 gene on the invasion and metastasis of endometrialcarcinoma cells.Methods:The ECC-1 endometrial carcinoma cell line (ER-positive) and KLE cell line (ER-negative) were selected andtransfected by Integrin beta1 siRNA plasmid (Integrin beta1 siRNA group), onsense sequence siRNA plasmid (nonsense sequence controlgroup) and empty vector (no load control group). The expressions of Integrin beta1 mRNA in each group were detected by using real-timePCR, the expressions of Integrin beta1, beta-catenin and C-Myc proteins in each group were tested by using Western blot, cell migration andinvasion of different groups were detected by using Transwell chamber, the cell proliferations of different groups were tested by usingMTT assay.Results:The relative expressions of Integrin beta1 mRNA and Integrin beta1 protein of the ECC-1 cells and KLE cells in theIntegrin beta1 siRNA group were relatively lower than those of the nonsense sequence control group and no load control group(P<0.05).The relative expressions of beta-catenin proteins and C-Myc proteins of the ECC-1 cells and KLE cells in the Integrin beta1 siRNA group werelower than those of the nonsense sequence control group and no load control group (P<0.05). The cell migration and invasion of ECC-1cells and KLE cells in the Integrin beta1 siRNA group were lower than those of the nonsense sequence control group and no load controlgroup (P<0.05). The A values of ECC-1 cells and KLE cells in the Integrin beta1 siRNA group were lower than those of the nonsensesequence control group and no load control group (P<0.05).Conclusion:Specific inhibition of Integrin beta1 gene expression could reducethe migration, invasion and proliferation of endometrial cancer cells, which might be related to the inhibition of Wnt signal pathway.