Recombinogenic activity of Pantoprazole? in somatic cells of
Drosophila melanogaster
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| Authors: | Jeyson Césary Lopes Nayane Moreira Machado Rosiane Soares Saturnino Júlio César Nepomuceno |
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| Institution: | 1.Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Campus Umuarama, Uberlândia, MG, Brazil.;2.Laboratório de Citogenética e Mutagênese, Centro Universitário de Patos de Minas, Patos de Minas, MG, Brazil. |
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| Abstract: | Pantoprazole® is one of the leading proton pump inhibitors (PPIs) used in
the treatment of a variety of diseases related to the upper gastrointestinal tract.
However, studies have shown an increased risk of developing gastric cancer,
intestinal metaplasia and hyperplasia of endocrine cells with prolonged use. In the
present study, the somatic mutation and recombination test (SMART) was employed to
determine the mutagenic effects of Pantoprazole on Drosophila
melanogaster. Repeated treatments with Pantoprazole were performed on
72-hour larvae of the standard (ST) and high bioactivation (HB) crosses at
concentrations of 2.5, 5.0, and 10.0 μM. In addition, doxorubicin (DXR) was
administered at 0.4 mM, as a positive control. When administered to ST descendants,
total number of spots were statistically significant at 2.5 and 5.0 μM
concentrations. For HB descendants, a significant increase in the total number of
spots was observed among the marked transheterozygous (MH) flies. Through analysis of
balancer heterozygous (BH) descendants, recombinogenic effects were observed at all
concentrations in descendants of the HB cross. In view of these experimental
conditions and results, it was concluded that Pantoprazole is associated with
recombinogenic effects in Drosophila melanogaster. |
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| Keywords: | Drosophila melanogaster mutagenicity proton pump inhibitors Pantoprazole recombinogenicity SMART |
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