Oxazine Dyes. I. Celestine Blue B with Iron as a Nuclear Stain

It is suggested that celestine blue B can stain as a colloidal dispersion, the nuclear specificity of which is controlled by the pH. The staining solution is prepared by adding 0.5 ml of concentrated H₂SO₄ to 1 gm of celestine blue B and dissolving the resultant granular mass in 100 ml of 2.5% ferric alum containing 14 ml of glycerol. Sections of amphibian, avian, and mammalian tissue placed for 1 min in this solution and then rinsed in water show as sharp nuclear staining as that usually produced by hematoxylin. A wide variety of fixatives is permissible. Overstaining is not possible within reasonable limits of exposure and no differentiation nor bluing is required. Both the staining solution and stained slides are stable.