Carnitine Inhibits Hydrolysis of Inositol Phospholipids Induced by Activation of Metabotropic Receptors |
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Authors: | Llansola Marta Felipo Vicente |
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Institution: | (1) Instituto de Investigaciones Citológicas de la Fundación Valenciana de Investigaciones Biomédicas, Amadeo de Saboya, 4, 46010 Valencia, Spain |
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Abstract: | We previously found that carnitine prevents glutamate neurotoxicity and that this effect is mediated by activation of metabotropic glutamate receptors. We show now that carnitine inhibits the hydrolysis of inositol phospholipids induced by different agonists of metabotropic glutamate receptors (tACPD; (±)-1-aminocyclopentane-trans-1,3-dicarboxylic acid; DHPG, (R,S)-3,5-dyhydroxyphenylglycine or S4C3HPG, (S)-4-carboxy-3-hydroxyphenylglycine). The EC50 was ca. 170 M and the inhibition was complete at 1 mM carnitine. Carnitine also inhibits completely hydrolysis of inositol phospholipids induced by arterenol (agonist of adrenoceptors) and only partially (ca. 50%) that induced by carbachol (agonist of muscarinic receptors). Carnitine did not inhibit phospholipase C activity but inhibits partially (43%) the hydrolysis of inositol phospholipids induced by direct activation of G proteins with AIP-4. The results reported indicate that carnitine inhibits the hydrolysis of inositol phospholipids induced by activation of metabotropic receptors likely by interfering the function of some types of G proteins. |
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Keywords: | Carnitine G proteins metabotropic glutamate receptors inositol phospholipid hydrolysis metabotropic receptors |
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