Influence of the composition of the fusion medium on the yield of electrofused yeast hybrids |
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Authors: | R. Schnettler U. Zimmermann |
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Affiliation: | Lehrstuhl für Biotechnologie der Universität Würzburg, Röntgenring 11, D-8700 Würzburg, F.R.G. |
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Abstract: | Abstract Electrofusion between cells of yeast strains with different genetic markers in isotonic sorbitol solutions leads to high yields of hybrids when 0.1 mM Ca2+ and 0.5 mM Mg2+ salts are aded. On average, 1000–2000 hybrids are obtained when electrofusion is performed (in a helical chamber) compared to a yield of about 40–120 in the absence of these bivalent cations. A further increase in yield can be achieved by the addition of 1 mg/ml albumin, which results in up to 4000 hybrids per experimental run. The entire fusion process leads to very reproducible results in the presence of these substances. |
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Keywords: | Electrodiaphoresis electrofusion protoplast Saccharomyces cerevisiae |
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