Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes |
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Authors: | Dan Röhme Howard Fox Bernhard Herrmann Anna-Maria Frischauf Jan-Erik Edström Paul Mains Lee M. Silver Hans Lehrach |
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Affiliation: | 1. European Molecular Biology Laboratory Postfach 10 22 09 6900 Heidelberg, Federal Republic of Germany;2. Cold Spring Harbor Laboratory Cold Spring Harbor, New York, 11724 USA;3. Department of Anatomy University of California, San Francisco San Francisco, California, 94143 USA |
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Abstract: | Fragments of the proximal half of mouse chromosome 17 including the t-complex region were microdissected from metaphase spreads. DNA was isolated from a pool of such fragments, and was cloned on microscale. Individual clones were used to probe genomic digests of DNA from a pair of Chinese hamster cell lines with or without mouse chromosome 17, and livers of congenic inbred lines of mice carrying wild-type and/or t-haplotype forms of chromosome 17. The data obtained indicate that 95% of the low copy number microclone inserts recognize DNA sequences present on mouse chromosome 17. It has been possible to use one-third of these clones to identify restriction-fragment-length polymorphisms between wild-type and t-haplotype DNA on a congenic background. These results demonstrate that these clones have been derived from the t-complex or regions closely linked to it. Clones of this type should provide starting points for a molecular analysis of this region of the mouse genome. |
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