Monitoring the biological treatment of anthracene-contaminated soil in a rotating-drum bioreactor |
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Authors: | D K Banerjee P M Fedorak A Hashimoto J H Masliyah M A Pickard M R Gray |
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Institution: | (1) Department of Chemical Engineering, University of Alberta, T6G 2G6 Edmonton, Alberta, Canada;(2) Department of Microbiology, University of Alberta, T6G 2E9 Edmonton, Alberta, Canada |
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Abstract: | A 2-kg-capacity rotating-drum reactor was used for biological conversion of nearly insoluble organic contaminants in soil. The rotating motion allowed effective operation at a solids content of over 60% by weight. A mixed bacterial culture was used to degrade anthracene that had been impregnated into a representative high-clay soil. The activity of the culture was sustained over a period of months in repeated batch operation, in which fresh soil was inoculated with 20% spent slurry from the previous run. Maximum degradation rates of 100–150 mg anthracene (kg soil)–1 day–1 were achieved throughout the experiments. Evolution of carbon dioxide from the bioreactor showed that degradation and mineralization of anthracene occurred simultaneously, and that 55% of the anthracene was mineralized. When the culture was switched from anthracene as sole carbon source to a mixture of three polynuclear aromatic hydrocarbons, the culture was able to degrade each of these in the sequence: anthracene, phenanthrene and finally pyrene. |
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