Changes in the novel orphan, C5a receptor (C5L2), during experimental sepsis and sepsis in humans |
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Authors: | Huber-Lang Markus Sarma J Vidya Rittirsch Daniel Schreiber Heike Weiss Manfred Flierl Michael Younkin Ellen Schneider Marion Suger-Wiedeck Heidemarie Gebhard Florian McClintock Shannon D Neff Thomas Zetoune Firas Bruckner Uwe Guo Ren-Feng Monk Peter N Ward Peter A |
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Affiliation: | Department of Traumatology, Hand and Reconstructive Surgery, University of Ulm Medical School, Germany. |
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Abstract: | Sepsis is associated with extensive complement activation, compromising innate immune defenses, especially in neutrophils (PMN). Recently, a second C5a receptor (C5L2) was detected on PMN without evidence of intracellular signaling. The current study was designed to determine changes in C5L2 in blood PMN during sepsis. In vitro exposure of PMN to C5a, but not to fMLP, led to reduced content of C5L2. Following cecal ligation and puncture-induced sepsis in rats, PMN demonstrated a time-dependent decrease in C5L2. In vivo blockade of C5a during experimental sepsis resulted in preservation of C5L2. Similarly, PMN from patients with progressive sepsis showed significantly reduced C5L2 expression (n = 26), which was virtually abolished in patients who developed multiorgan failure (n = 10). In contrast, sepsis survivors exhibited retention of C5L2 (n = 12/13). The data suggest that C5L2 on PMN diminishes during sepsis due to systemic generation of C5a, which is associated with a poor prognosis. |
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