Use of Acadian marine plant extract powder from Ascophyllum nodosum in tissue culture of Kappaphycus varieties |
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Authors: | Anicia Q Hurtado Dianne Aster Yunque Keneth Tibubos Alan T Critchley |
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Institution: | (1) Aquaculture Department, Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, 5021, Philippines;(2) Integrated Services for the Development of Aquaculture and Fisheries, McArthur Highway, Tabuc Suba, Jaro, Iloilo, 5000, Philippines;(3) Acadian Seaplants Limited, 30 Brown Avenue, Dartmouth, NS B3B 1X8, Canada |
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Abstract: | Three varieties of Kappaphycus alvarezii (Kapilaran, KAP), Tambalang purple (PUR), Adik-adik (AA), and one variety of Kappaphycus striatum var. sacol (green sacol (GS) were used to determine the efficiency of Acadian marine plant extract powder (AMPEP) as a culture medium
at different concentrations, for the regeneration of young plants of Kappaphycus varieties, using tissue culture techniques for the production of seed stock for nursery and outplanting purposes for the
commercial cultivation of carrageenophytes. A shorter duration for shoot formation was observed when the explant was treated
with AMPEP + Plant Growth Regulator (PGR = PAA + zeatin at 1 mg L−1) compared to AMPEP when used singly. However, four explants responded differently to the number of days required for shoot
formation. The KAP variety took 46 days to form shoots at 3–4 mg L−1 AMPEP + PGR; while PUR required 21 days at 3–5 mg L−1 AMPEP and 3–4 mg L−1 AMPEP + PGR. AA required 17 days at 3–5 mg L−1 AMPEP and AMPEP + PGR; and GS 25 days at 1 mg L−1 AMPEP + PGR. It was observed that among the four explants used, PUR and AA initiated shoot formation with the use of AMPEP
only at higher concentrations (3–5 mg L−1) after a shorter period. Only PUR responded positively to ESS/2 for shoot initiation. The use of AMPEP alone and/or in combination
with PGR as a culture medium in the propagation of microplantlets using tissue culture technique is highly encouraging. |
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