Analysis of human erythrocyte glucose 6-phosphate dehydrogenase isozymes by isoelectric focusing in polyacrylamide gel |
| |
| Authors: | Vazken M. Der Kaloustian Shirine H. Idriss-Daouk Ruwayda T. Hallal Z. L. Awdeh |
| |
| Affiliation: | 1. Department of Pediatrics, American University of Beirut, Beirut, Lebanon
2. Nutrition Research Laboratories, American University of Beirut, Beirut, Lebanon
|
| |
| Abstract: | The method of isoelectric focusing in polyacrylamide gel was used to separate G6PD isozymes in crude hemolysates of human, rabbit, and rat erythrocytes. G6PD (B) from erythrocytes of a normal human male donor revealed six bands of activity. Their mean isoelectric points, using pH 3–10 and 5–8 range empholytes, were pI 7.04 for band I, pI 6.60 for band II, pI 6.37 for band III, pI 6.11 for band IV, pI 5.94 for band V, pI 5.79 for band VI. G6PD from rabbit and rat erythrocytes revealed completely different multiple band patterns. The method of isoelectric focusing in polyacrylamide gel is presented as a new way of detecting G6PD isozyme patterns. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
