Freeze-substitution improves the ultrastructural preservation of legume root hairs |
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Authors: | Robert W Ridge |
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Institution: | (1) Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi, 305 Ibaraki |
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Abstract: | The freeze-substitution method was applied toVicia hirsuta root hairs to test its effectiveness in improving preservation of the cell ultrastruture. Freeze-substitution almost certainly
represents more faithfully the structure of the hair cell. A previously unreported ‘pyriformis’ vesicle is described. Also
unique to freeze-substituted material are coated secretory vesicles; a smooth plasma membrane profile; mitochondrial ribosomes;
long microfilament bundles which are associated with vesicles, mitochondria, coated pits and coated vesicles; microtubule-associated
filaments; well-preserved coated vesicles and coated pits with enclosing rings; a pliciform nucleus. The results are discussed
in context of previous reports using conventional fixation techniques. |
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Keywords: | Coated pits and vesicles Freeze-substitution Legume root hair ultrastructure Microfilaments Pliciform nucleus Pyriformis vesicles |
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