NMR resonance assignment of DnaE intein from Nostoc punctiforme |
| |
Authors: | Kimmo Heinämäki Jesper S Oeemig Janica Djupsjöbacka Hideo Iwaï |
| |
Institution: | (1) Research Program in Structural Biology and Biophysics, Institute of Biotechnology, University of Helsinki, P.O. Box 65, Helsinki, 00014, Finland |
| |
Abstract: | DnaE intein from Nostoc punctiforme (Npu) is one of naturally occurring split inteins, which has robust protein splicing activity. Highly efficient trans-splicing activity of NpuDnaE intein could widen various biotechnological applications. However, structural basis of the efficient protein splicing activity is poorly understood. As a first step toward better understanding of protein trans-splicing mechanism, we present the backbone and side-chain resonance assignments of a single chain variant NpuDnaE intein as determined by triple resonance experiments with 13C,15N]-labeled protein. |
| |
Keywords: | Protein splicing Intein Protein trans-splicing |
本文献已被 SpringerLink 等数据库收录! |
|