Identification of a novel elite genotype for in vitro culture and genetic transformation of cotton |
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Authors: | S Jin X Zhang Y Nie X Guo S Liang H Zhu |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, P.R. China |
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Abstract: | Hypocotyls of cotton (Gossypium hirsutum L.) cultivars cv. YZ-1, Coker 312 and Coker 201 were inoculated on Murashige and Skoog callus induction medium. YZ-1 exhibited
a very high regeneration potential, with 81.9 % of the explants inoculated differentiated into embryogenic callus within 8–10
weeks. During the process of callus maintenance (subculture for 1 to 3 years), the total embryos number in Coker 312 and Coker
201 calli dropped sharply, and the percentage of embryo germination decreased. On the contrary, the callus of YZ-1 consistently
maintains a high frequency of plant regeneration after long-time subculture. Transgenic kanamycin-resistant calli of Coker
201 partially lost the ability of somatic embryogenesis and plant regeneration. The stress produced by the transformation
procedure slightly affected somatic embryogenesis and plant regeneration of YZ-1, which showed minimum loss of plant regeneration
ability. |
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Keywords: | Gossypium hirsutum regeneration potential somatic embryogenesis |
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