超声激活血卟啉对S180 肿瘤细胞表面EGFR 表达量的影响

目的：探讨超声激活血卟啉处理S180肿瘤细胞后膜表面EGFR表达量的变化。方法：将腹水瘤细胞随机分为四组，U组和UH组细胞分别于频率1．8MHz、2．0W／cm^2的超声装置中照射3min，并分别在1h3h5h后取材，应用免疫组化方法在光镜下观察EGFR的表达情况。结果：1h、3h取材，U组和UH组平均光密度值明显低于Cr组和H组，UH组最低。而5h取材时，UH组平均光密度值显著下降，其它组基本无变化。结论：提示在高频低强度处理下，随着时间的延长，超声激活HpD对EGFR的抑制作用增加，显示可能是基因调控使EGFR表达下调，从而使肿瘤细胞增殖减慢。

Effect of ultrasound activating hematophorphyrin on the expression of EGFR in ascites S180 cell membrane

Objective: To study the change of expression of EGFR in ascites S180 cell membrane during the process of ultrasound activating hematophorphyrin.Methods: After being divided into four groups in random(U,UH,CT and H group),the tumor cells of U and UH groups have been exposed to ultrasound at 1.80MHz and 2.0W/cm2 for 3min by using HpD.After 1h,3h and 5h,the tumor cells in the four groups were observed under optic microscope.Results: EGFR in CT an H groups has a higher expression in S180 ascites cells after 1h and 3h exposed to ultrasound together with HpD.EGFR in UH group has a distinct decrease after 5h exposed to ultrasound together with HpD,while other three groups have no changes.Conclusion: High frequency and low intensity ultrasound activating hematophorphyrin could inhibit EGFR,slow down the multiplication of cancer cell,which suggests that gene may be involved in regulating the expression of EGFR in SDT.