Cytotoxic and genotoxic effects of sodium hypochlorite on human peripheral lymphocytes in vitro |
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Authors: | Süleyman Gül Asu Savsar Zeynep Tayfa |
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Institution: | (1) Department of Biology, Faculty of Sciences and Arts, University of Kafkas, Kars, Turkey |
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Abstract: | Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and
genotoxic effects of sodium hypochlorite were investigated by the cytokinesis-block micronucleus assay and chromosomal aberration
analysis on human peripheral lymphocytes in vitro. A significant increase in chromosomal aberration frequency was observed
in all treatments of NaOCl (0.030, 0.065, 0.100, 0.25, 0.5, 1, 2, 4 μg/mL) at 24 and 48 h compared with the negative control
and mitomycin C (MMC, 0.3 μg/mL), which was used as a positive control. NaOCl significantly increased the frequency of micronuclei
in a dose dependent manner. The results showed that there was a significant correlation between NaOCl concentration and chromosomal
aberration, micronuclei frequency, necrotic cells, apoptotic cells and binucleated cells. |
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Keywords: | Sodium hypochlorite Chromosomal aberrations (CA) Micronucleus (MN) assay Necrotic cell Apoptotic cell |
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