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应用微卫星标记分析野生红光熊蜂蜂王的一妻多夫水平
引用本文:彭文君,罗其花,安建东,李长春,刘晓东,马玉珍.应用微卫星标记分析野生红光熊蜂蜂王的一妻多夫水平[J].昆虫知识,2012,49(5):1125-1131.
作者姓名:彭文君  罗其花  安建东  李长春  刘晓东  马玉珍
作者单位:1. 中国农业科学院蜜蜂研究所 北京 100093
2. 北京市密云县园林绿化局 北京 101500
基金项目:农业部引进国际先进农业科学技术“948”滚动项目(2006-G19)和国家自然科学基金项目(30972149).
摘    要:为了摸清自然条件下红光熊蜂Bombus ignitus Smith的一妻多夫水平,本实验采用B118、B11、B96、B124和B126微卫星位点,对来自5群野生红光熊蜂(A、B、C、D和E)的工蜂和雄蜂样本进行了分析。结果推导出:在A、B、C、D和E群体的工蜂基因型中,来自父本的基因型分别有3、2、3、4和3种,即与母本蜂王交配的雄蜂数量分别是3、2、3、4和3只。表明在自然条件下,红光熊蜂同其他蜜蜂科的蜂种一样,也存在一妻多夫现象,且与蜂王交配的雄蜂数量平均为3只。

关 键 词:微卫星  红光熊蜂  一妻多夫水平

Levels of polyandry of wild Bombus ignitus queens base on microsateilite DNA markers
PENG Wen-Jun , LUO Qi-Hua , AN Jian-Dong , LI Chang-Chun , LIU Xiao-Dong , MA Yu-Zhen.Levels of polyandry of wild Bombus ignitus queens base on microsateilite DNA markers[J].Entomological Knowledge,2012,49(5):1125-1131.
Authors:PENG Wen-Jun  LUO Qi-Hua  AN Jian-Dong  LI Chang-Chun  LIU Xiao-Dong  MA Yu-Zhen
Institution:1. Institute of Apicuhural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 2. Miyun County Bureau of Landscape and Forestry, Beijing 101500, China)
Abstract:Samples of queens, workers and drones from five colonies of wild Bombus ignitus Smith were assessed at the Bllg, BI1, B96, B124 and B126 microsatellite loci to analyse levels of polyandry. 3, 2, 3, 4 and 3 father genotypes, respectively, were found in workers from the five colonies, indicating that the respective queens had each mated with 3, 2, 3, 4 and 3 males. These results suggest that polyandry is common in B. ignitus colonies as with other species of Anidae. and that the average number of males with which each queen had mated was 3.
Keywords:microsatellite  Bombus ignitus  levels of polyandry
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