百草枯对蜜蜂寿命及抗氧化酶基因表达影响

本研究用200、400和600 mg/L的百草枯饲喂刚出房的意大利蜜蜂Apis mellifera ligustica Spinola工蜂,检测其对工蜂寿命及抗氧化酶基因Sod 1和Sod 2表达的影响,并以饲喂蔗糖作为对照组.结果表明:随着百草枯浓度的增加,工蜂的寿命极显著下降(P＜0.01)；抗氧化酶基因Sod 1的相对表达量,随着百草枯浓度的增加而增加,其中对照组Sod 1基因的相对表达量显著低于各百草枯处理组(P ＜0.05);Sod 2基因的相对表达量,随着百草枯浓度的增加,先增加后减少,其中600 mg/L百草枯处理组Sod 2基因的相对表达量显著低于对照组和低剂量百草枯处理组(P＜0.05).

Effects of paraquat on lifespan enzyme genes in worker and level of expression of antioxidant bees of Apis meUifera ligustica

We investigated the effects of paraquat on lifespan and the level of expression of mRNA for the antioxidant enzyme genes Sod 1 and Sod 2 in newly emerged worker bees of Apis mellifera ligustica Spinola. Paraquat was given at doses of 200 rag/L, 400 mg/L and 600 mg/L. Bees in the control group were fed sucrose. The results showed that lifespans of honey bees decreased with the increase of paraquat concentration（ P 〈 O. 01 ）. The relative level of expression of the antioxidant enzyme gene Sod I increased linearly with the increase of paraquat concentration and it was significantly lower in the control group than in the paraquat-treated groups （ P 〈 0. 05）. In contrast, the relative level of expression of the antioxidant enzyme gene Sod 2 increased initially but then decreased with the increase of paraquat concentration. The level of expression of this gene was significantly lower in the high-dosage paraquat-treated group （600 mg/L） than that in both the control group and the low-and middle-dosage paraquat-treated groups （P 〈 0.05）.