| 芫菁斑蝥素对几种动物蛋白磷酸酶2A的抑制作用 |
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| 引用本文： | 李晓飞,侯晓晖,陈祥盛,娄方明,刘云. 芫菁斑蝥素对几种动物蛋白磷酸酶2A的抑制作用[J]. 昆虫知识, 2012, 49(5): 1226-1230 |
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| 作者姓名： | 李晓飞 侯晓晖 陈祥盛 娄方明 刘云 |
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| 作者单位： | 1. 遵义医学院基础医学院 遵义 563003
2. 贵州大学昆虫研究所 贵阳 550025 |
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| 基金项目： | 国家自然科学基金（30800111）、教育部科学技术研究重点项目（209109）、贵州省优秀科技教育人才省长专项基金（黔省专合字[2010]48号）. |
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| 摘 要： | 研究斑蝥素对马、兔、猪、鸡、眼斑芫菁Mylabris cichorii(L.)等几种动物体内PP2A的抑制作用。将斑蝥素作用于马、兔、猪、鸡、眼斑芫菁等几种动物体内的PP2A,采用蛋白磷酸酶抑制法-比色法测定斑蝥素对PP2A的抑制率。斑蝥素对马、兔、猪等哺乳动物体内的PP2A有明显的抑制作用,IC50分别为0.07、0.12、0.23 g/L;斑蝥素对鸡体内的PP2A有一定的抑制作用,而对于眼斑芫菁体内PP2A抑制作用不明显。马、兔、猪体内的PP2A对斑蝥素很敏感,而鸡和眼斑芫菁体内的PP2A对斑蝥素较不敏感。
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| 关 键 词： | 眼斑芫菁 斑蝥素 蛋白磷酸酶2A 抑制中浓度 毒性 |
Inhibitory effect of cantharidin on PP2A in several kinds of animals |
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| LI Xiao-Fei , HOU Xiao-Hui , CHEN Xiang-Sheng , LOU Fang-Ming , LIU Yun. Inhibitory effect of cantharidin on PP2A in several kinds of animals[J]. Entomological Knowledge, 2012, 49(5): 1226-1230 |
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| Authors: | LI Xiao-Fei HOU Xiao-Hui CHEN Xiang-Sheng LOU Fang-Ming LIU Yun |
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| Affiliation: | 1. Basic Medicine School, Zunyi Medical College, Zunyi 563003, China; 2. Institute of Entomology, Guizhou University, Guiyang 550025, China) |
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| Abstract: | We used the eolorimetric protein phosphatases inhibition assay to study the inhibitory effect of cantharadin extracted from meloids on PP2A in horses, rabbits, pigs, chickens and Mylabris eichorii （ L. ）. CanthaHdin had an inhibitory effect on horse, pig and rabbit PP2A with IC50 values of 0.07 g/L, 0.12 g/L and 0.23 g/L, respectively. The results suggest that although horses, pigs and rabbits are sensitive to cantharidin, chickens and M. cichorii are not. |
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| Keywords: | Mylabris cichorii cantharidin PP2A IC50 toxicity |
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