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| Calcofluor White M2R与Sytox Green双重染色法鉴别蜜蜂微孢子虫 | |
| 引用本文： | 秦浩然,李继莲,和绍禹,吴杰.Calcofluor White M2R与Sytox Green双重染色法鉴别蜜蜂微孢子虫[J].昆虫知识,2012,49(5):1392-1396. |
| 作者姓名： | 秦浩然 李继莲 和绍禹 吴杰 |
| 作者单位： | 1. 农业部授粉昆虫生物学重点实验室 中国农业科学院蜜蜂研究所 北京 100093;云南农业大学 东方蜜蜂研究所 昆明 650201 2. 农业部授粉昆虫生物学重点实验室 中国农业科学院蜜蜂研究所 北京 100093 3. 云南农业大学 东方蜜蜂研究所 昆明 650201 |
| 基金项目： | 国际科技合作项目（2009DFA32600）、国家自然基金（30972149,30700606）、农业部公益性行业（农业）科研专项课题（201203080-4）和国家蜂产业技术体系建设专项（CARS-45）. |
| 摘 要： | 东方蜜蜂微孢子虫(Nosema ceranae)是一种广泛寄生于东方蜜蜂Apis cerana,西方蜜蜂Apis mellifera和熊蜂Bombus Latreille上的寄生虫,对蜜蜂和熊蜂的危害较大,进而影响养蜂业的发展。本实验采用荧光染色试剂Calcofluor White M2R与核酸染料Sytox Green双重染法来鉴别蜜蜂或熊蜂体内的N.ceranae及孢子的存活状态。结果得出,在荧光显微镜下可见死孢子被染上黄绿色荧光,活的呈现蓝白色荧光,而寄主细胞、细菌、病毒等不被染色。这是一种快速有效鉴别N.ceranae及其死活的方法,从而判定蜜蜂或熊蜂体内的微孢子虫在是否具有侵染活性,对微孢子虫的研究及药物防治具有重要作用。 |
| 关 键 词： | 东方蜜蜂微孢子虫 荧光染色 Calcofluor White M2R SytoxGreen |
Detection and identification of Nosema ceranae by dual fluorescent staining with Calcofluor White M2R and Sytox Green |
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| QIN Hao-Ran , LI Ji-Lian , HE Shao-Yu , WU Jie.Detection and identification of Nosema ceranae by dual fluorescent staining with Calcofluor White M2R and Sytox Green[J].Entomological Knowledge,2012,49(5):1392-1396. | |
| Authors: | QIN Hao-Ran LI Ji-Lian HE Shao-Yu WU Jie |
| Institution: | QIN Hao-Ran, LI Ji-Lian, HE Shao-Yu,WE Jie ( 1. Key Laboratory of Pollinating Insect Biology of the Ministry of Agriculture, Institute of Apicuhural Research, Chinese Academy of Agricultural Science, Xiangshan, Beijing 100093, China; 2. Eastern Bee Research Institute of Yunnan Agrieuhural University, Heilongtan, Kunming 650201, China) |
| Abstract: | Nosema ceranae is a microsporidian parasite of Apis cerana, Apis mellifera and bumblebees that has an adverse effect on pollination, especially of fruits and vegetables, and apiculture. In this study, Calcofluor White M2R and Sytox Green stains were used to discriminate between live and dead N. ceranae. The results show that dead N. ceranae spores had yellow-green fluorescence whereas live spores fluoresced white - blue. This method allows easy detection and identification of live and dead N. ceranae and should therefore contribute to further research on N. ceranae and its treatment. |
| Keywords: | Nosema ceranae fluorescent staining Calcofluor White M2R Sytox Green |
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