Assignment of the backbone carbonyl resonances in 15N-labelled proteins with 13C at natural abundance by a 2D triple-resonance correlation technique期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Assignment of the backbone carbonyl resonances in 15N-labelled proteins with 13C at natural abundance by a 2D triple-resonance correlation technique

Authors:	Søren M Kristensen Morten Dahl Sørensen Jens J Led

Institution:	(1) Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen Ø, Denmark

Abstract:	Summary A 2D NMR experiment for assignment of backbone carbon resonances in small and medium-sized ¹⁵N-labelled proteins with ¹³C at natural abundance is presented. The experiment is a two-dimensional variant of the HNCO triple-resonance experiment and is demonstrated by application to a 6 kDa protein at relatively low concentration (2 mM) and temperature (30°C). The experiment is particularly suitable for assignment of carbonyl resonances.

Keywords:	Carbonyl resonance assignment Natural abundance ¹³C 2D NMR Triple resonance HNCO
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