| 绿木霉SM1蛋白诱导拟南芥防御反应以及激发活性氧途径研究 |
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| 引用本文： | 张泰龙,朱洁伟,陈捷. 绿木霉SM1蛋白诱导拟南芥防御反应以及激发活性氧途径研究[J]. 菌物学报, 2014, 33(6): 1302-1312. DOI: 10.13346/j.mycosystema.140502 |
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| 作者姓名： | 张泰龙 朱洁伟 陈捷 |
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| 作者单位： | 上海交通大学农业与生物学院 农业部都市农业（南方）重点开放实验室 微生物代谢国家重点实验室 上海 200240 |
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| 基金项目： | Supported by the National Natural Science Foundation of China(No.30971949);the Special Fund for Agro-scientific Research in the Public Interest(No.200903056);the Special Fund of National Maize Production System(No.CARS-02);the Basic Work Project Suppoting Science and Technology Research(No.2014FY120900) |
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| 摘 要： | SM1蛋白是由绿木霉Trichoderma virens产生的一种富含半胱氨酸的小蛋白，能够作为激发子激发植物防御反应。研究了SM1蛋白对拟南芥Arabidopsis thaliana生长及诱导抗性的作用。结果表明高浓度（>10μg/mL）SM1蛋白液抑制拟南芥的生长，低浓度SM1蛋白液则不影响生长；SM1能诱导拟南芥对细菌性叶斑病Pseudomonas syringae pv. tomato DC3000的抗性，引起拟南芥叶片过氧化氢的积累。SM1蛋白处理后，拟南芥叶片中植物防御反应相关基因PDF1.2、LOX2和活性氧酶基因 SOD、POD等表达显著上升，说明SM1在激活植物的JA/ET和ROS途径中发挥着重要作用。研究为进一步研究SM1诱导植物抗性的机理提供了基础。
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| 关 键 词： | 激发子 SM1蛋白 绿木霉 拟南芥 茉莉酸/乙烯途径 活性氧 |
SM1 protein produced by Trichoderma virens induces defense responses and activates ROS signaling pathway in Arabidopsis |
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| ZHANG Tai-Long,ZHU Jie-Wei,CHEN Jie. SM1 protein produced by Trichoderma virens induces defense responses and activates ROS signaling pathway in Arabidopsis[J]. Mycosystema, 2014, 33(6): 1302-1312. DOI: 10.13346/j.mycosystema.140502 |
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| Authors: | ZHANG Tai-Long ZHU Jie-Wei CHEN Jie |
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| Affiliation: | School of Agriculture and Biology, Key Laboratory of Urban Agriculture (South), State Key Laboratory of Microbial Metabolism, Shanghai Jiaotong University, Shanghai 200240, China |
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| Abstract: | A cysteine-rich small protein secreted by Trichoderma virens, designated SM1, acts as an elicitor for triggering plant defense reactions. We investigated the effects of the SM1 on growth and induced resistance in Arabidopsis thaliana. The results showed that high concentrations of SM1 (>10μg/mL) inhibited the growth of A. thaliana, while the low concentrations were capable of inducing resistance to bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and causing the accumulation of hydrogen peroxide without interfering the growth of A. thaliana. After SM1 treatment, the expression of the defense-related genes (PDF1.2 and LOX2) and the antioxidant scavenging enzyme genes (SOD and POD) were significantly up-regulated in A. thaliana leaves, indicating that SM1 plays an important role in activating JA/ET (jasmonic acid/ethylene) and ROS (reactive oxygen species) signaling pathways in plants. This research laid a foundation for the further study on the mechanism of SM1-induced plant resistance. |
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| Keywords: | elicitor SM1 protein Trichoderma virens Arabidopsis thaliana jasmonic acid/ethylene (JA/ET) pathway reactive oxygen species (ROS) |
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