The desaturation and elongation of linolenic acid and eicosapentaenoic acid by hepatocytes and liver microsomes from rainbow trout (Oncorhynchus mykiss) fed diets containing fish oil or olive oil |
| |
| Institution: | 4. Laboratory of Epidemiology and Population Sciences, National Institute on Aging, Bethesda, MD;5. Department of Health Sciences, VU University Medical Center, Amsterdam, The Netherlands;6. Department of Epidemiology and Biostatistics and the EMGO+ Institute for Health and Care Research, VU University Medical Center, Amsterdam, The Netherlands;7. Division of Public Health Sciences, Cancer Prevention Program, Fred Hutchinson Cancer Research Center, Seattle, WA;8. Icelandic Heart Association Research Institute, Kopavogur, Iceland;9. Faculty of Medicine, University of Iceland, Reykjavik, Iceland;1. ETH Zurich, Institute of Agricultural Sciences, Universitätstrasse 2, 8092 Zurich, Switzerland;2. University of Zurich, Vetsuisse Faculty, Department of Farm Animals, Winterthurerstrasse 260, 8057 Zurich, Switzerland;3. University of Zurich, Vetsuisse Faculty, Clinic for Zoo Animals, Exotic Pets and Wildlife, Winterthurerstrasse 260, 8057 Zurich, Switzerland;1. Department of Vascular Diseases, Ljubljana University Medical Center, Zalo?ka cesta 7, SI-1000 Ljubljana, Slovenia;2. Department of Endocrinology, Diabetes and Metabolic Diseases, Ljubljana University Medical Center, Zalo?ka cesta 7, SI-1000 Ljubljana, Slovenia;3. Diabetes & Me, Diabetes Center, ?idovska cesta 1, SI-1000 Ljubljana, Slovenia;1. Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China;2. Department of Dermatology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China;3. Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China;1. Singapore Institute for Clinical Sciences, Agency for Science Technology and Research (A?STAR), Singapore;2. Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore;3. Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;4. Department of Maternal Fetal Medicine, KK Women''s and Children''s Hospital, Singapore;5. Duke-NUS Medical School, Singapore;6. Department of Diagnostic and Interventional Imaging, KK Women''s and Children''s Hospital, Singapore;7. Department of Paediatrics, KK Women''s and Children''s Hospital, Singapore;8. Division of Paediatric Allergy, Immunology & Rheumatology, Khoo Teck Puat - National University Children''s Medical Institute, National University Health System, Singapore;9. Liggins Institute, University of Auckland, Auckland, New Zealand;10. MRC Lifecourse Epidemiology Unit; Southampton, United Kingdom;11. NIHR Southampton Biomedical Research Centre, University of Southampton and University Hospital Southampton NHS Foundation Trust, Southampton, United Kingdom;12. Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Southampton, United Kingdom;13. Clinical Nutrition Research Centre (CNRC), Singapore Institute for Clinical Sciences, Centre for Translational Medicine, Singapore;14. Department of Pediatrics and of Epidemiology and Biostatistics, McGill University Faculty of Medicine, Montreal, Quebec, Canada;15. U1153 Epidemiology and Biostatistics Sorbonne Paris Cité Research Centre (CRESS), Early Origin of the Child''s Health and Development (ORCHAD) Team, Inserm, Villejuif, France;p. Univ Paris Descartes, Villejuif, France;q. Faculty of Pharmacy, Univ Paris-Sud, Université Paris-Saclay, Châtenay-Malabry, France;r. Division of Paediatric Endocrinology and Diabetes, Khoo Teck Puat, National University Children''s Medical Institute, National University Health System, Singapore;1. Hospital del Mar Medical Research Institute, Barcelona, Spain;2. CIBER of Pathophysiology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain;3. Department of Epidemiology and Public Health, University College London, UK;4. Division of Surgery and Interventional Sciences, University College London, London, UK;5. Centre for Fertility and Health (CeFH), Norwegian Institute of Public Health, Oslo, Norway;6. Blanquerna School of Health Sciences, Universitat Ramon Llull, Barcelona, Spain;7. August Pi i Sunyer Biomedical Research Institute, Barcelona, Spain;8. MRC Lifecourse Epidemiology Unit, University of Southampton, UK;9. Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, UK |
| |
| Abstract: | The products of desaturation and elongation of 1?14C] 18:3(n ? 3) and 1?14C]20:5(n ? 3) were studied using hepatocytes and microsomes prepared from livers of trout maintained on diets containing either olive oil or fish oil, to establish the extent to which the formation of 22:6(n ? 3) was enhanced in the absence of dietary 22:6(n ? 3) and to investigate the pathway(s) of conversion of 18:3(n ? 3) and 20:5(n ? 3) to 22:6(n ? 3). Levels of 20:5(n ? 3) and 22:6(n ? 3) in the total lipid of hepatocytes from trout fed olive oil were 20-fold and 10-fold, respectively, lower than in cells from trout fed fish oil. For both dietary groups, 1?14C]18:3(n ? 3) was incorporated into hepatocyte lipid to a greater extent than 1?14C]20:5(n ? 3). Almost 70% of the total radioactivity from 1?14C]18:3(n ? 3) was recovered in hepatocyte triacylglycerols, whereas radioactivity from 1?14C]20:5(n ? 3) was recovered almost equally in neutral lipids (52%) and polar lipids (48%). The products of desaturation and elongation from both labelled substrates were esterified mainly into hepatocyte polar lipids, whereas elongation products of 1?14C]18:3(n ? 3) were preferentially incorporated into neutral lipids. Radioactivity recovered in the 22:6(n ? 3) of polar lipids of hepatocytes from trout fed olive oil, from both 14C substrates, was approximately double that in hepatocytes from trout fed fish oil. No radioactivity from either 1?14C]18:3(n ? 3) or 1?14C]20:5(n ? 3) was incorporated into 22:6(n ? 3) by microsomes isolated from livers from either group of fish and incubated in the presence of acetyl-CoA, malonyl-CoA, NADH, NADPH, ATP and coenzyme A. However, significant radioactivity was recovered in 24:5(n ? 3) and 24:6(n ? 3) from 1?14C]20:5(n ? 3) and more radioactive 24:6(n ? 3) accumulated in microsomes from trout fed olive oil than from trout fed fish oil. The results establish that the formation of 22:6(n ? 3) from both 18:3(n ? 3) and 20:5(n ? 3) in hepatocytes of rainbow trout is stimulated by omitting 22:6(n ? 3) from the diet and are consistent with the biosynthesis of 22:6(n ? 3) in trout liver cells proceeding via 24:5(n ? 3) and 24:6(n ? 3) intermediates. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
