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The counterion influence on cationic lipid-mediated transfection of plasmid DNA
Affiliation:1. Department of Environmental Engineering, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, 615-8540, Kyoto, Japan;2. Research Center for Environmental Quality Management, Kyoto University, 1-2 Yumihama, Otsu, 520-0811, Shiga, Japan;3. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin-kawahara-cho, Sakyo-ku, 606-8507, Kyoto, Japan;1. Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, College of Veterinary Medicine and Zootechny, São Paulo, Brazil;2. Department of Pathology, University of São Paulo, College of Veterinary Medicine and Zootechnics, São Paulo, Brazil;3. Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil;4. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
Abstract:A panel of DOTAP analogs was prepared by altering the anionic counterion that accompanies the trimethylammonium polar domain. The transfection of plasmid DNA into NIH3T3 cells and mouse lung was examined using the counterion analogs. The in vitro transfection activity decreased as follows: DOTAP · bisulfate > trifluoromethanesulfonate ∼ iodide ∼ bromide > dihydrogenphosphate ∼ chloride ∼ acetate > sulfate. A similar activity trend was observed in vivo.
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