ABCB1 基因单核苷酸多态性rs2235048 与氯吡格雷抵抗的相关关系

目的:探讨ABCB1基因单核苷酸多态位点rs2235048在中国汉族人群中的分布及其与氯吡格雷抵抗(Clopidogrelresistance,CR)发生的相关关系。方法:采用光学比浊法测定20μmol/L ADP诱导的残余血小板聚集率(Residual plateletagglutination,RPA)。当RPA≥70%时,即为CR。所有入选患者分为CR组和氯吡格雷非抵抗组(Non-clopidogrel resistance,NCR)。采用焦磷酸测序法测定ABCB1基因rs2235048单核苷酸多态位点在CR组和NCR组的基因型及等位基因分布频率。结果:ABCB1基因rs2235048多态在CR组和NCR组基因型分布频率符合Hardy-Weinberg平衡。在CR组和NCR组中,ABCB1基因rs2235048多态的基因型频率分布无统计学差异(P=0.527,X2=1.281);T、C等位基因频率在两组间分布频率也没有统计学差异(P=0.740,OR=0.958,95%CI=0.742～1.236)。结论:对PCI术后服用氯吡格雷的冠心病患者进行分析发现,ABCB1基因单核苷酸多态位点rs2235048与冠心病患者CR的发生无相关关系。

The Association between ABCB1 Gene rs2235048 Single Nucleotide Polymorphism and Clopidogrel Resistance

Objective: To investigate the frequnency of gene polymorphism in rs2235048 of ATP-binding cassette subfamily B member l transporter(ABCB1) gene and to elucidate the correlation between this polymophism and the occurrence of clopidogrel resistance(CR).Methods: Clopidogrel response was assessed by posttreatment ADP 20μmol/L-induced platelet aggregation.Clopidogrel resistance was defined by RPA(RPA≥70%),and all the enrolled 800 coronary artery diseases(CAD) patients after percutaneous coronary intervention(PCI) were divided into CR group and Non-CR(NCR) group.Genotypes were determined by polymerase chain reaction(PCR) and the sequences of rs2235048 of ABCB1 gene were analyzed.Results: The genotype frequencies in rs2235048 of ABCB1 gene conformed well to the Hardy-Weinberg equilibrium for both CR group and NCR group.Between the two groups,the genotype and allele frequency was similar(P=0.527 and 0.740,respectively).Conclusions: Based on the genetic analysis in patients after PCI with clopidogrel therapy,there was no correlation between ABCB1 rs2235048 polymorphism and clopidogrel resistance.