胰岛素小鼠皮下注射的降血糖作用研究

目的:了解胰岛素皮下注射后不同时间对小鼠血糖的影响。方法:采用正规胰岛素制剂0.5U/ml予小鼠按0.054ml/10g皮下注射后,观察在注射后30分钟、60分钟、90分钟、120分钟和150分钟后血糖的变化,并在注射150分钟后予10%葡萄糖灌胃,观察小鼠30分钟和60分钟后的血糖变化。结果:皮下注射各剂量的胰岛素均能引起小鼠血糖降低,其中以注射90分钟后血糖下降最为明显,高剂量胰岛素降血糖作用最明显。给予葡萄糖水灌胃后血糖有所升高,但是高剂量恢复比较慢。结论:0.5U/ml胰岛素按0.108ml/10g皮下注射会引起小鼠血糖过低,不利于实验数据的收集,0.027ml/10g和0.054ml/10g剂量比较合适。

The Effect of Blood Sugar Level After Insulin Skin-Poping in Mice

Objective: To study the effect of different blood sugar level at different time after insulin skin-pop in mice.Methods: Inject(skin-popi) the mice with regular insulin(0.5U/ml,0.054ml/10g),After 30 minutes,60 minutes,90 minutes,120 minutes and 150 minutes to detect the blood sugar.And at the time after injection 150 minutes later,fill the mice with 10% glucose,To study the blood sugar level of the mice.Results: Skin-popping the different dosage of insulin could reduce the blood sugar in mice.It was most obvious in high dose insulin and at 90 minutes after injection.The blood sugar were increasing after filling the 10% glucose,but it increased more slowly in high dose group.Conclusions: the insulin after skin-poping in 0.55u/ml,0.108ml/10g in mice,blood surger level reduced,so,it was not good for the collection of experimental data,but a dose of 0.027ml/10g and 0.054ml/10g would be better choice.