骨桥蛋白在胰腺癌中的表达及其临床意义

目的:研究骨桥蛋白OPN在胰腺癌中的表达水平及其与临床病理特征的关系。方法:采用RT-PCR和免疫组织化学方法分别检测50例胰腺癌手术切除标本和20例癌旁正常胰腺组织中OPNmRNA及其蛋白水平的表达。结果:胰腺癌组织中OPNmRNA高表达率为90%(45/50)明显高于其在癌旁正常胰腺组织中的表达15%(3/20),差异有统计学意义(P<0.01);OPN蛋白的阳性率为86.0%(43/50),明显高于癌旁正常胰腺组织中的表达30%(6/20),差异有统计学意义(P<0.01);OPN在胰腺癌组织中表达与其淋巴结转移明显相关(P<0.05)。结论:OPN在胰腺癌中的过表达对胰腺癌的生长、浸润及转移起重要作用。

Expression of Osteopontin in Pancreatic Cancer and Its Clinical Significance

Objective: To study the mRNA and protein expression levels of osteopontin in pancreatic cancer and the relationship with clinic pathological features.Methods: Amount of 50 cases of pancreatic cancer surgical specimens and 20 cases of adjacent normal tissue were studied.The OPN mRNA and protein expression levels were detected by RT-PCR and immunohistochemistry respectively.Results: RT-PCR analysis showed that the OPN mRNA were highly expressed in pancreatic tissues(45/50,90 %),which was significantly higher than in adjacent normal tissues(3/20,15 %)(P<0.01).Immunohistochemistry analysis demonstrated that the OPN protein was high expressed in pancreatic tissues(43/50,86 %),which was significantly higher than in adjacent normal tissue(6/20,30 %)(P<0.01).The expression levels of OPN in pancreatic cancer were obviously associated with lymph node metastasis(P<0.05).Conclusion: The OPN was overexpressed in pancreatic cancer,which maybe played important roles in the growth,invasion and metastasis of cancer.